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Category : Epitope Tags and Fusion Protein Antibodies :   beta Galactosidase Antibodies : Affinity Purified Chicken anti-beta Galactosidase

Item Affinity Purified Chicken anti-beta Galactosidase More Details :



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Host Chicken
Catalog Number CGAL-45A-Z
Sales Price $140.00
Quantity 0.1 mg
Physical State Liquid
Buffer Phosphate Buffered Saline pH 7.2
Preservative 0.1% sodium azide
Immunofluorescence 2 ug/ml
Storage 4 C
Procedure Chickens were immunized with purified beta-galactosidase from Escherichia coli. After multiple immunizations in Freunds adjuvant, eggs were collected and delipidated. Antibodies were solid phase absorbed then immunoaffinity purified using purified beta-galactosidase immobilized on a solid phase. The anti-beta galactosidase concentration was determined by extinction coefficient : O.D. 1.32 at 280nM equals 1.0 mg
Specification Recommended starting dilution of anti-beta galactosidase for IF is 0.5 ug/ml. Less than 1% reactivity with E. Coli.
Western Blots 1:500 - 1:2,000.
ELISA coating 1:200-1:500
Application For Research and Manufacturing Only; Not For Use in Diagnostic Applications
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