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Category : Antibodies to Human Proteins :   Antibodies to Human Kappa : HRP Conjugated Chicken anti-Human Kappa b+f

Item HRP Conjugated Chicken anti-Human Kappa b+f More Details :



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Host Chicken
Catalog Number CKBF-80P
Sales Price $150.00
Quantity 1.0 mg
Physical State Liquid
Buffer 50 mM HEPES pH 7.0, 0.1M NaCl with 0.2% BSA
Preservative 0.01% Thimerosal
Storage 4 C
Procedure Chickens were immunized with highly purified Human kappa light chains and Fab kappa. After multiple immunizations in Freunds adjuvant eggs were collected and an IgY fraction was prepared. The antibodies were immunoaffinity isolated and conjugated with horseradish peroxidase (HRP).
Specification Before conjugation the antibody was shown to react with all human immunoglobulin classes possessing kappa chains and free kappa light chains by IEP and gel diffusion techniques. It does not react with kappa chains. Conjugate is suitable for ELISA, Western blotting and immunohistochemistry, For ELISA we recommend dilutions of between 1:20,000 and 1:100,000. Optimal conjugate working dilutions should be determined for your particular assay conditions.
Western Blots 1:100-1:5000
ELISA 1:15,000-1:30,000
Application For Research and Manufacturing Only; Not for Use in Diagnostic Applications



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